mouse cxcl12 Search Results


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mouse cxcl12 duoset elisa  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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sdf 1  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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mouse cxcl12 sdf 1 alpha quantikine elisa kit  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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anti sdf 1 antibody  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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recombinant mouse cxcl12 460 sd  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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cat mcx120 detection limit of 156 pg ml  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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mouse cxcl12 antibody  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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recombinant mouse cxcl12  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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high sensitivity mouse cxcl12 sdf 1 elisa kits  (R&D Systems)
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in <t>CXCL12</t> secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.
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MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in CXCL12 secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.

Journal: bioRxiv

Article Title: Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury

doi: 10.1101/239194

Figure Lengend Snippet: MSC compatibility with PRM scaffolds. (A) Characterization of PRM scaffolds by scanning electron microscopy. (B) BrdU and phalloidin immunostaining of MSCs on coverslips or PRM scaffolds. Cells were able to adhere and incorporate BrdU into DNA (proliferating cells) in either condition (n = 2, experiment in triplicates). Scale bar = 100 μm. (C) After seven days in culture, MTT assay showed similar survival of MSCs when cells were cultured on coverslips or PRM scaffolds (p > 0.05, Student’s t test, n = 5). (D) TUNEL assay showed that MSCs cultured on coverslips or PRM scaffolds did not undergo apoptosis (n = 2, experiment in triplicates). Scale bar = 100 μm. (E) MSCs cultured on coverslips or PRM scaffolds were induced to differentiate in osteocytes and adipocytes. After 3 weeks, cells were stained with Oil Red (staining of lipid vesicles in red) or Alizarin Red (staining of calcium deposits in red). MSCs cultured on PRM scaffolds preserved multipotency (n = 2, experiment in triplicates). Upper scale bar = 50 μm; lower scale bar = 200 μm. (F) PRM induced a 50% increase in CXCL12 secretion by MSCs ( p < 0.05, Student’s t test, n = 5). MSCs: mesenchymal stem cells, PRM: polymeric rough microfibers, BrdU: 5-bromo-2’-deoxyuridine, MTT: 3-(4,5-dimethylthiazol-yl)2,5-diphenyltetrazolium bromide, TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, DAPI: 4’,6-diamidino-2-phenylindole.

Article Snippet: CXCL 12 in conditioned media was quantified using Mouse CXCL12 DuoSet ELISA (R&D systems, Minneapolis, USA).

Techniques: Electron Microscopy, Immunostaining, MTT Assay, Cell Culture, TUNEL Assay, Staining, End Labeling